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how to calculate aet for extractable and leachable study??


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  1. AET is the threshold at which analytical techniques can detect the compounds.
    AET for parenteral products can be calculated using following formulas:
    1) for liquid injectables

    AET (extractable) = (SCT*LC*Vc)/(MDD*Ve)

    AET (leachable) = ( SCT*LC)/MDD

    Where,
    AET = Analytical evaluation threshold
    in microgram/ ml of extraction solution for extractable and microgram/ml of drug product for leachable
    SCT = 1.5 microgram/day for parenterals
    LC = Label claim of drug product mg/ml (i.e. drug product in ml shall be considered)
    Vc = Volume of drug product in one container i.e. fill volume of drug product in ml
    MDD = maximum daily dose of drug product in mg/day considering mg of main component
    Ve = volume of extraction solvent used in ml

    2) for solid injectables i.e. sterile powder, lyo products

    AET (extractable) = (SCT*LC)/(MDD*Ve)

    AET (leachable) = ( SCT*LC)/(MDD*Vr)

    Where, SCT = 1.5 microgram/day for parenterals
    LC = Label claim of drug product mg/vial (i.e. drug product in mg of main component shall be considered)
    MDD = maximum daily dose of drug product in mg/day considering mg of main component
    Ve = volume of extraction solvent used in ml
    Vr = volume of dilution/ reconstitution solution used in ml

    Note: due to uncertainties of anaytical techniques, 50% of AET shall be reccomend as reporting threshold

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